Abstract: Viticulture is currently experiencing a gradual shift to more sustainable production practices. Many producers see in this shift an opportunity to increase their sales, especially in a context which is greatly influenced by the reduction in wine sales due to the world economic crisis. Hence, both organic and biodynamic viticulture have begun to be applied in many vineyards as alternative attractive agricultural techniques. Nevertheless, it remains unclear which are the exact environmental benefits (or drawbacks) of applying these techniques for numerous environmental impacts, such as climate change or toxicity. Therefore, the main goal of this study is to perform an environmental evaluation using Life Cycle Assessment (LCA) for three different viticulture techniques within a single appellation (Ribeiro, NW Spain): biodynamic cultivation sites, conventional vineyards and an intermediate biodynamic-conventional wine-growing plantation (i.